Published 01/01/2006
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Characterization of Insulin-Loaded Alginate Nanoparticles Produced by Ionotropic Pre-Gelation Through DSC and FTIR Studies

R.António , F.Domingos , S.Bruno , V.Francisco

Keywords
Introduction
Nanoparticles made of polyelectrolytes complexation have shown potential for use as drug delivery systems. Polyelectrolyte complexes are formed when oppo-sitely charged polyelectrolytes are mixed and interact via electrostatic interactions. Chitosan and alginate are polyca-tion and polyanion polyelectrolytes, respectively, that can be used to form a polyelectrolyte complex and to deliver proteins , peptidic drugs and DNA.
Resume
The influence of the pH and stoichiometry relationship between polyelectrolytes providing individual par-ticles with a nano-scale size was assessed by photon correlation spectroscopy and scanning electron microscopy. Insulin polyelectrolyte interactions at varying pH and polyelectrolytes stoichiometry were assessed by differential scanning calorimetry and Fourier-transform infrared studies. Thermograms of insulin-loaded nanoparticles originated shifts on same unloaded nanopar-ticle peaks and suggested polyelectrolytesprotein interactions at pH around 4. 55. 0.
Method
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Results
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Conclusion
Insulin-loaded nanoparticles were prepared by ionotropic pre-gelation of alginate with calcium chloride followed by complexation between alginate and chitosan. The influence of the pH and stoichiometry relationship between polyelectrolytes providing individual particles with a nano-scale size was assessed by photon correlation spectroscopy (PCS) and scanning electron microscopy (SEM). Insulin-polyelectrolyte interactions at varying pH and polyelectrolytes stoichiometry were assessed by differential scanning calorimetry (DSC) and Fourier-transform infrared (FTIR) studies. Individual and smaller sizing nanoparticles, around 800 nm, were obtained at pH 4.7 with an alginate:chitosan mass ratio of 6:1. Thermograms of insulin-loaded nanoparticles originated shifts on same unloaded nanoparticle peaks and suggested polyelectrolytes-protein interactions at pH around 4.5-5.0. FTIR spectra of insulin-loaded nanoparticles showed amide absorption bands characteristic of protein spectra and revealed the formation of new chemical entities.http://www.sciencedirect.com/science/article/B6TFD-4JKYTNH-1/1/f2878177436b8ca802aa251efe948a9
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